MeDIP-Seq 생명정보 분석

1. Data filtering: Data filtering includes removing adaptors, contamination and low-quality reads from raw reads.

2. Standard bioinformatics analysis

1. Reads alignment

2. Distribution of Uniquely Mapped Reads

2.1 Reads Distribution in Each Chromosome (only for species with complete chromosomes information)

2.2 Genome Coverage Distribution Across Sequencing Depth

2.3 Distribution of CG, CHG and CHH sites Across Sequencing Depth

2.4 Distribution of MeDIP-Seq Reads in Different GC Content Regions

2.5 Reads Distribution in Different Genome Regions

2.6 Distribution in Genome Region, Upstream2k and Downstream2k Across Sequencing Depth

3. Peak scanning and genome-wide distribution

3.1 Peak Scanning

3.2 Peak Length Distribution Analysis

3.3 Peak GC Content Analysis for Each Sample

3.4 Analysis of Peak Correlated Genes

3.5 Peak Distribution in Different Gene Regions

4. Difference analysis of multi-samples

4.1 Differential Analysis

4.2 Go Enrichment Analysis and KEGG Pathway Analysis