TotalOmics

태그 유전자 발현 해독 생명정보 분석

1. Data filtering: Data filtering includes removing adaptors, contamination and low-quality reads from raw tags.

2. Standard bioinformatics analysis:

   1. Assessment of sequencing (Statistics of tag alignment, Sequencing saturation analysis, Distribution of clean tag copy number, Statistics of clean tag alignment, Statistics of reference genes

   2. Gene expression annotation

   3. Differential gene expression analysis (Screening of differentially expressed genes (DEGs), Experimental repeatability analysis of DEGs)

   4. Anti-sense transcripts annotation

   5. Novel transcripts detection (reference genome needed)

   6. Expression pattern analysis of DEGs (Client to determine what conditions to meet to perform cluster analysis, for example, "hormone treatment in 2 hours, 4 hours, 8 hours showed a significant difference of gene expression")

   7. Gene ontology analysis of DEGs

   8. Pathway enrichment analysis of DEGs

   9. Protein-protein interaction network analysis (protein-protein interaction database of the target species needed).