TotalOmics
전사체 정량 해독 생명정보 분석
1. Data filtering: Data filtering includes removing adaptors, contamination and low-quality reads from raw reads.
2. Standard bioinformatics analysis:
1. Assessment of sequencing (Statistics of raw reads, Sequencing saturation analysis, Analysis of the distribution of reads on reference gene)
2. Gene expression annotation
3. Differential gene expression analysis (Screening of differentially expressed genes(DEGs), Experimental repeatability analysis of DEGs. Two or more samples should be provided; if the alignment has been conducted more than 10 times, it will be charged as the custom bioinformatics anaysis)
4. Expression pattern analysis of DEGs (Client to determine what conditions to meet to perform cluster analysis, For example, "hormone treatment in 2 hours, 4 hours, 8 hours showed a significant difference of gene expression")
5. Gene ontology analysis of DEGs
6. Pathway enrichment analysis of DEGs
7. Protein-protein interaction network analysis (protein-protein interaction database of the target species needed).
