TotalOmics
스몰 RNA 전사체 해독 생명정보 분석
1. Data filtering
Remove adaptors, low quality reads as well as contaminants to get clean reads and summarize the
length distribution of small RNA.
2. Standard bioinformatics analysis:
1. Analyze common and specific sequences between two samples
2. Explore small RNA distribution across selected genome (reference genome is required for the analysis. The test sample/samples can only be used for alignment to one reference genome; if more than one reference alignments needed, additional fee will be charged.)
3. Identify rRNAs, tRNAs, snRNAs, etc. by alignment to Rfam and Genbank databases
4. Identify repeat associated small RNAs (repeat annotation information of the reference genome should be provided)
5. Identify degradated fragment of mRNAs (gene annotation information of the reference genome should be provided)
6. Identify known miRNAs by alignment to designated part of miRBase (the known miRNAs should be specified, e.g. known miRNAs from human, all miRNAs from plants or animals, or known miRNAs from certain species)
7. Annotate small RNAs into several categories based on priority
8. Predict novel miRNAs and their secondary structures by Mireap from unannotated small RNAs
9. Analyze the expression pattern of known miRNAs
10. Family analysis of known miRNAs (Latin name of the species should be provided)
