TotalOmics



스몰 RNA 전사체 해독 샘플 조건

• Sample condition: integrated total RNA samples.

                                    Avoid protein contamination during RNA isolation.

• Sample quantity: general requirements: total RNA ≥ 15 μg;

                                  for plasma/serum or used for co-IP: total RNA ≥ 150 ng (≥ 5ml for serum sample);

                                  for < 200 nt small RNA isolated from mirVana™ miRNA Isolation Kit: total RNA ≥ 1 μg.

• Sample concentration: for general requirements: concentration ≥ 400 ng/μl;

                                             for plasma/serum or used for co-IP: concentration ≥ 5 ng/μl;

                                             for < 200 nt small RNA isolated from mirVana™ miRNA Isolation Kit supplied: concentration ≥ 20 ng/μl.

• Sample purity: OD260/280=1.8~2.2;

                              for animal samples, RNA 28S:18S≥1.5, RIN≥7.0;

                              for plant samples, no requirements for RNA 28S:18S and RIN values.