선도 유전체 해독 샘플 조건

or diploid genomic DNA samples with a heterozygosity rate less than 0.5%

Sample quantity (for a single library preparation): for short-insert libraries, ≥10 μg;

for 2 Kb~10 Kb large-insert libraries, ≥60 μg;

for 20 Kb large-insert libraries, ≥90 μg;

the total sample quantity for whole genome sequencing is about 1 mg

for 2Kb~10Kb large-insert libraries > 150 ng/ul;

for 20Kb large-insert libraries > 200 ng/ul

Sample purity: OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination

Sample quality: genomic DNA should be intact. If there were large-insert libraries with an insert size larger than 5 Kb to be constructed, major bands of genomic DNA should be 23 Kb or above in the result of electrophoresis. For the result of pulsed-field gel electrophoresis, the main bands of DNA should be more than 40 Kb

If tissue samples are provided, plant samples should be etiolated seedlings, callus, tissue culture or tender leaves, and animal samples should be tissues with less fat such as muscles and blood; it would be better to provide samples from the same individual, which may decrease impact of individual differences on bioinformatics analysis. If the sex determination is XY, XX type is preferable; if the sex determination is ZW, ZZ type is preferable.

As for the heterozygous genome with a heterozygosity rate more than 0.5% or polyploidy genome, it should be treated on a case by case basis.