메타 16S rRNA 해독 생명정보 분석

1. 16S rDNA metagenomics survey:

1. Illumina QC report

2. Remove the low-quality reads (poly-ATGC) and adaptors

3. Overlap the paired-end reads to form tags

4. Remove the primer

5. Compute alpha diversity for Complexity Analysis in single sample

6. Species classification analysis by BlastN, OTU analysis

7. Species (or OTU) abundance analysis

8. Phylogenetic analysis based on OTU variance (Under the condition that the sample with low richness of OTU)

Whether the subsequent comparative analysis could be carried on is based on 16S rDNA metagenomics survey results.

2. Multi-samples comparative analysis of 16S rDNA:

The following analysis could be provided based on species (or OTU) information.

1. Primary comparative analysis

1.1 Generate profiling table

1.2 Compute beta diversity for Complexity Analysis in multi-samples

1.3 Principal component analysis

1.4 Cluster analysis based on Species (or OTU) information

2. Advanced comparative analysis

2.1 Screening significant factors (Species or OTU) correlated with sample grouping

3. Personalized analysis: We can also perform customized analysis to meet requirements of specific projects.