TotalOmics
균류 유전체 선도 해독 샘플 조건
- Sample condition: DNA samples
- Sample quantity(for a single library preparation): for short-insert libraries, ≥ 9 μg;
for 2Kb~6 Kb large-insert libraries, ≥ 60 μg;
for PCR-free libraries constructed for the genome with high or low GC content, ≥ 45 μg;
the total quantity is determined by the experimental strategy of the type and number of libraries to be constructed;
- Sample concentration: for short-insert libraries, ≥ 50 ng/μl;
for 2 Kb~6 Kb large-insert libraries, ≥ 150 ng/μl;
- Sample purity: OD260/280=1.8~2.0.
