Totalomics Solutions

TotalOmics

 균류 유전체 선도 해독 샘플 조건


  • Sample condition: DNA samples


  • Sample quantity(for a single library preparation): for short-insert libraries, ≥ 9 μg;

                                  for 2Kb~6 Kb large-insert libraries, ≥ 60 μg;

                                  for PCR-free libraries constructed for the genome with high or low GC content, ≥ 45 μg;

                                  the total quantity is determined by the experimental strategy of the type and number of libraries to be constructed;


  • Sample concentration: for short-insert libraries, ≥ 50 ng/μl;

                                             for 2 Kb~6 Kb large-insert libraries, ≥ 150 ng/μl;


  • Sample purity: OD260/280=1.8~2.0.